microarray printing robot  (Bio-Rad)

Journal: Micromachines

Article Title: Portable Fluorescence Microarray Reader-Enabled Biomarker Panel Detection System for Point-of-Care Diagnosis of Lupus Nephritis

doi: 10.3390/mi16020156

Figure Lengend Snippet: ( A ) Layout of the biomarker microarray (BMA) slide, showing the arrangement of capture antibodies and controls within each well. ( B ) Illustration of the BMA slide design with 16 wells and its alignment within the slide holder for analysis. The numbers along the side correspond to the row numbers of each well, while the numbers along the bottom represent the column positions of the slide.

Article Snippet: The slides were prepared using a non-contact microarray printing robot (sciFLEXARRAYER S3; Scienion GmbH, Berlin, Germany), which printed capture antibodies for each biomarker in triplicate at a controlled drop volume of 450 ± 20 pL.

Techniques: Biomarker Assay, Microarray

Journal: Plants

Article Title: Banana Peel ( Musa ABB cv. Nam Wa Mali-Ong) as a Source of Value-Adding Components and the Functional Properties of Its Bioactive Ingredients

doi: 10.3390/plants13050593

Figure Lengend Snippet: Heatmap diagram of glycoactive ingredients recovered by CDTA, NaOH, H 2 O, and cellulase reagents ( A ); loading plot ( B ) and biplot ( C ) of the monoclonal antibodies in comprehensive microarray polymer profiling. The green circle in C symbolises the potential correlation between the glycoactive ingredients and the antibody.

Article Snippet: Subsequently, the samples were printed onto a nitrocellulose membrane using a microarray printing robot (Marathon Argus, Arrayjet, Roslin, UK).

Techniques: Bioprocessing, Microarray, Polymer

Journal: PLoS ONE

Article Title: An Integrated Peptide-Antigen Microarray on Plasmonic Gold Films for Sensitive Human Antibody Profiling

doi: 10.1371/journal.pone.0071043

Figure Lengend Snippet: glass. A) Schematic of a 4-plex histone peptide microarray containing unmodified and acetylated Histone H2B and H3 peptides immobilized on gold plasmonic and glass substrates for interacting with a mixture of 2 commercial antibodies against the acetylated H2B and acetylated H3 peptides in a solution (right photo). B) Top panel: fluorescence images of histone peptide microarrays on gold plasmonic substrate probed in titration with commercial antibodies targeting acetylated H2B and H3 peptide. Bottom Panel: identical peptide microarrays on streptavidin coated glass slides probed with the same commercial antibodies as top panel. C) and D) are calibration curves for antibody quantification generated by averaging the integrated fluorescence intensity of secondary antibody-IRDye800 emission over the 5 duplicate microarray spots for each of the histone peptide at various concentrations of histone antibodies on plasmonic gold slide and streptavidin-glass slide. Error bars represent the standard deviation of the mean over the 5 duplicate assay features.

Article Snippet: The avidin coated gold slides (or commercial streptavidin coated glass slides) were loaded into a microarray printing robot (Bio-Rad) where 0.2 mg/ml biotin conjugated peptide in PBS supplemented with 4% glycerol was printed using solid pins (Arrayit) at 25°C and 60% humidity, resulting in microarray feature diameters of ∼200 μm.

Techniques: Peptide Microarray, Fluorescence, Titration, Generated, Microarray, Standard Deviation

Journal: PLoS ONE

Article Title: An Integrated Peptide-Antigen Microarray on Plasmonic Gold Films for Sensitive Human Antibody Profiling

doi: 10.1371/journal.pone.0071043

Figure Lengend Snippet: A) Peptide-antigen microarray layout including modified and unmodified histone H2A, H2B, H3, H4 peptides as well as whole antigens. Ac: acetylated; aa: amino acid; Me1: methylated; Me2: dimethylated; Me3: trimethylated; Ph: phosphorylated; K: Lysine; S: Serine. Number indicates amino acid position from the N-terminus of its corresponding histone proteins. Peptide sequences are listed in . B) Microarray imaging results of a SLE patient serum probed on a plasmonic gold substrate (left) vs. on a commercial streptavidin-glass substrate (right) shown in the same IRDye800 fluorescence intensity scale. C) Dynamic range of fluorescence signal reading among 20 SLE patients measured on each peptide and antigen spots on avidin/gold slide (upper panel) and commercial streptavidin/glass slide (lower panel). Each dot represents a fluorescence intensity measured on a peptide or antigen spot shown along the x-axis in the serum of each of the 20 SLE patients. A dot is drawn only when the fluorescence intensity is above the background by 2 standard deviation of the background signal.

Article Snippet: The avidin coated gold slides (or commercial streptavidin coated glass slides) were loaded into a microarray printing robot (Bio-Rad) where 0.2 mg/ml biotin conjugated peptide in PBS supplemented with 4% glycerol was printed using solid pins (Arrayit) at 25°C and 60% humidity, resulting in microarray feature diameters of ∼200 μm.

Techniques: Microarray, Modification, Methylation, Imaging, Fluorescence, Avidin-Biotin Assay, Standard Deviation

Journal: PLoS ONE

Article Title: An Integrated Peptide-Antigen Microarray on Plasmonic Gold Films for Sensitive Human Antibody Profiling

doi: 10.1371/journal.pone.0071043

Figure Lengend Snippet: A) Box plot of serum IgG antibody reactivity against several peptides and a whole antigen for 20 SLE patients and 20 healthy controls. Acetylated histone H2B peptides were found to be able to differentiate SLE patients and healthy controls (top box plots) together with whole H2B protein (bottom left box plot). While the H3 peptide with the 18th lysine methylated were found not capable of telling SLE patient from healthy control (bottom right box plot). B–D) Heatmaps displaying antibody reactivity to (B) histone peptides only, (C) whole antigens only, and (D) a combination of histone peptides and whole antigens that are identified capable of differentiating SLE patients and healthy controls with false discovery rate (q value) <0.001. The dashed lines are drawn to highlight the separation of SLE and healthy groups identified by using the average linkage Euclidean distance hierarchical clustering method. Color intensity of each grid in the heatmap reflected mean fluorescence intensity of corresponding peptide or antigen spot on the microarray for each SLE patient or healthy individual. In (B) and (C), several SLE patients (labeled in blue and purple color) are misplaced in the healthy group. These patients are grouped in the SLE side in (D) that profiles antibodies against both peptides and whole antigens. However, one healthy individual is mis-placed in the SLE group by this approach, reducing the specificity of this analysis.

Article Snippet: The avidin coated gold slides (or commercial streptavidin coated glass slides) were loaded into a microarray printing robot (Bio-Rad) where 0.2 mg/ml biotin conjugated peptide in PBS supplemented with 4% glycerol was printed using solid pins (Arrayit) at 25°C and 60% humidity, resulting in microarray feature diameters of ∼200 μm.

Techniques: Methylation, Fluorescence, Microarray, Labeling